Single Cell Omics Solutions for SARS-CoV-2 (COVID-19) Infection
Unlocking the mystery inside single cells.
Single Cell Omics Profiling of COVID-19 Reveals New Pathological Insights and Novel Therapeutic Candidates.
The ongoing SARS-CoV-2 (COVID-19) outbreak has taken thousands of lives and the number of infections is growing daily. It become the largest threat to public health. Single-cell omics technologies are valuable tools for the characterization of the transcriptional changes and gene expression differences after SARS-CoV-2 infection. As an unparalleled single cell omics service provider, SingleX provides tailored single-cell sequencing and analyses solutions to accelerate SARS-CoV-2 (COVID-19) research and therapy development.
At present, great advances have been made in SARS-CoV-2 research. Studies have indicated that SARS-CoV-2 mainly employs ACE2 as a receptor for cellular entry and this process depends on TMPRSS2 protease activity and cathepsin B/L activity as a substitute. Here, we provide single-cell RNA sequence (scRNA-seq) to comprehensively characterize the gene expression changes of different tissues/cells from COVID-19 patients. Through which, we can speculate the possible genes involved in the virus entry and conclude which cell type may be more vulnerable to SARS-CoV-2 infection.
By applying single-cell RNA sequence (scRNA-seq) and V(D)J information to comprehensively characterize the peripheral blood mononuclear cells from COVID-19 patients at different stages, we can get a high-resolution transcriptome landscape of blood immune subsets in the patients. These mirroring changes in blood and pinpoint cell-specific alterations are usually associated with the immune responses and severity of the disease. With scRNA-seq technology, we can combine immunophenotype, gene expression, and antigen receptor sequences, at single cell resolution. Immune profiling is of significant importance for immune repertoire analysis (TCR, BCR), antibody discovery, and immunotherapy exploration.
Emerging single-cell epigenomic methods have been developed to transform our knowledge of gene regulation, especially high-throughput single-cell assay for transposase accessible chromatin sequencing (ATAC-seq). Single-cell ATAC-seq can help us understand the sequence variation of ACE2 and changes in the proportions of cell subtypes in organs between species. Through analyses of ATAC-seq data, we can choose a best-fit animal model closest to humans to advance COVID-19 research.
Spatial gene expression analyses at single-cell resolution will reveal insights into the molecular organizations of cells and tissues, which is a key determinant of cell fate and behavior. Based on the SARS-CoV-2, researchers applied single-cell transcriptome analyses to study the spatial expression patterns of ACE2 in various tissues and cell types of the human body to clearly reveal the entry mechanism of the virus.
Single-cell CRISPR screening is a powerful technique that enables detecting the expression changes of the whole transcriptome at single-cell resolution. During infection, it unravels gene regulatory networks and enhancer-gene regulations on a large scale to combat pathogens. Now it has been used to target both the virus genome and host factors to identify genomic elements associated with virus infection, helping to clear viral reservoirs and prohibit virus infection or replication.
Protein variability at the single-cell level is critical for understanding the molecular mechanisms of cellular processes. In addition, it has regulatory roles in controlling cell fate during apoptosis induction or the proliferation/quiescence decision. Characterizing the quantity and activity of proteins during virus infection may give us new insights about the disease progression, cell differentiation and fate, and for targeted discovery and development of novel therapeutics, vaccines, and diagnostics.
Single-cell sequencing technologies and other high-throughput methods can characterize the heterogeneity at the mRNA level for human cells and viral RNA and the protein level. Single-cell immune profiling is being utilized to rapidly identify matched heavy chain and light chain antibody genes from B cells isolated from patients in the recovery stage, providing a powerful tool to discover neutralizing antibodies against SARS-CoV-2 and other coronaviruses.
- High-throughput single cell analysis
- High-quality data for faster decisions
- Simple experimental procedure and highly reproducible
- Excellent sensitivity and cell capture rate
SingleX provides a comprehensive suite of single cell omics solutions for SARS-CoV-2 (COVID-19). Our scientists are dedicated to designing innovative approaches to gain a multidimensional view of your research at single-cell resolution. If you are interested in our services, please feel free to contact us to learn how we can be involved in your COVID-19 research.