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Cellular metabolomics is an important reference for assessing phenotypic heterogeneity. When the living environment of a cell population changes drastically, the difference between different cell individuals will be the key to determining cell fate. At present, the theory and application of metabolomics at the single-cell level are gradually improved. SingleX has established a relatively mature platform for single-cell metabolomics technology, which is expected to achieve more complete metabolome coverage, better and faster identification of metabolites and improvement of nondestructive testing.

Single Metabolomics

In recent years, the potential of single-cell omics in the diagnosis and treatment of diseases has begun to attract attention, and the number of research topics and medical projects related to single-cell genomics, transcriptome, proteomics, and metabolomics has increased significantly. A metabolome is defined as a set of small molecule metabolites found in a particular cell, organ or organism. Due to the highly dynamic nature of cellular metabolism, single-cell metabolomics can be used to discover chemical strategies by which cells respond to chemical or environmental stresses. Compared to other single-cell omics, metabolomics describes cell function more directly and dynamically, but this is undoubtedly the most difficult to measure. This is because the metabolites have a large structural diversity, a wide dynamic range, the inability to amplify metabolites, and the use of fluorescent labels may distort their normal function, allow dynamic response to the environment in a short period of time.

Prepare the samples for single-cell MALDI-MS analysis. Fig.1 Prepare the samples for single-cell MALDI-MS analysis.

Single Cell Metabolomics Analysis Technologies

To test individual cell responses to stimuli or environmental stress, SingleX provides an important reference for customer research projects by accurately analyzing cell conditions and comparing these data with the metabolomics database. Our analytical methods for identifying single cell metabolites are as follows:

  • Mass spectrometry (MS) - Our MS assays are unlabeled, highly sensitive and informative, and are one of the most widely used methods for ultra-sensitive detection of many metabolites at single-cell level.
  • Mass spectrometry imaging (MSI) - MSI is capable of detecting and identifying a broad range of metabolites and is often applied to tissue sections, where a cell-by-cell distinction is feasible.
  • Fluorescence-based detection - Fluorescence microscopy is an excellent, sensitive, non-destructive, widely used method for imaging single cells that is typically used in conjunction with fluorescent labels to provide chemically specific parameters. With modern image processing, information can be accessed directly in a high throughput manner.
  • Vibrational spectroscopy - Vibrational spectroscopy can be used to distinguish certain metabolites in single cells. As opposed to detection through fluorescent tags, vibrational spectroscopy is label-free and thus amenable to spectroscopically active compounds (e.g., pigments) as long as they are present in sufficiently high concentration.

To explore more about our single cell solutions, please feel free to contact us by email or send us an inquiry to find an end-to-end solution.


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